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Pull-Down Assay: A Key Technique for Protein-Protein Interaction Analysis - Creative Proteomics

The fluorescence quenching values, (F 0 − F)/F 0 , showed a linear dynamic range for the concentrations of GSH from 0.25 to 16 µM, which is sensitive to detect the milli-molar concentrations of endogenous GSH in most mammalian cells

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Other Kme readers, including HP1 ( Here, we describe the development of a high-throughput assay for rapid, in vitro determination of where a Kme reader may bind in the proteome